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KMID : 0380620140460030328
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Korean Journal of Food Science and Technology
2014 Volume.46 No. 3 p.328 ~ p.333
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Screening of Lactic Acid Bacteria for Strong Folate Synthesis and Optimization of Fermentation
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Du Kyung-Min
Park Se-Jin
Park Myung-Soo
Ji Geun-Eog
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Abstract
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Folate is a water-soluble vitamin B that is required for the synthesis of amino acids and nucleic acids. It plays an important role in cell division and cell growth in several living organisms. The purpose of this study was to screen strong folate-synthesizing bacteria and to optimize their culture conditions for folate production. Folate production was quantified by microbiological assays by using folate-dependent strain Lactobacillus rhamnosus KCTC 3237. Folate derivatives were identified by LC-MS/MS. Of the 65 strains of bifidobacteria and lactobacilli tested, L. plantarum Fol 708 demonstrated the greatest ability to produce folate. Its optimal pH for folate production was 5.5 in a pH-controlled, labscale fermenter. Coculturing L. plantarum Fol 708 with L. brevis GABA 100 in a milk medium enhanced the level of folate produced in comparison to culturing L. plantarum Fol 708 alone.
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KEYWORD
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folate, microbiological assay, fermented-food, lactobacilli, co-culture
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